Wigan, who are looking to win a third successive Super League title, will face Warrington in Las Vegas on 1 March.
Easter Weekend, which had traditionally seen teams play twice across the four days until 2022, will feature the first Rivals Round of the season, with those fixtures reversed in round 25 at the back-end of the campaign.
The final round of matches will be played between 18 and 21 September and the elimination play-offs will take place the following weekend.
The Grand Final will be held at Old Trafford on Saturday, 4 October.